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In contrast, the percentage of ⁶⁵Zn in the intestine and kidney of Lm dams was about twice that of N dams. The percentage of the ⁶⁵Zn dose recovered in the intestine, liver, and kidney of N pups nursed by LM dams was markedly lower than those of N pups nursed by N dams. Transport of ⁶⁵Zn to milk of Lm dams was about 50% compared to milk of N dams. ⁶⁵Zn content of milk and tissues of dams and tissues of pups was measured. They also received 800 μg Zn/ml in their drinking water to preclude short term, terminal zinc deficiency in the neonates nursed by Lm dams. To examine tissue Zn distribution and Zn transport to milk and pups, 1 μCi of ⁶⁵Zn was administered ip to. The genetic defect may cause a shift in the tissue Zn distribution in Lm dams since their milk has a 34-45% lower Zn concentration than milk of normal (N) dams.

The lethal milk (Lm) mutation is autosomal recessive in C57BL/6J mice and causes Zn deficiency in pups nursed by Lm dams. The nurses recognize the instrument's importance for the allocation of professionals, organization, and care planning. It was concluded that there was a consensus and agreement among the nurses that the instrument was complete, easy to understand and applicable, but was very time consuming. The Kappa level of agreement among the nurses was found to be excellent for most care areas (69.0%). Nine nurses applied the instrument to 63 NB, with two of the nurses working simultaneously in each of the nursery's five sectors. The study was conducted at the nursery of a public hospital in São Paulo. This observational study evaluates the reliability of an NB classification instrument.

Evaluation occurs in the delivery room, taking into consideration the following factors: birth weight, gestational age, and behaviors that define the clinical picture and disease. In most maternity units, the physician classifies, evaluates, and determines which unit will receive the newborn (NB) after birth.